| ORIGINAL RESEARCH |
|
| Year : 2017 | Volume
: 8
| Issue : 4 | Page : 94-99 |
|
|
Antimicrobial efficacy of silver nanoparticles with and without different antimicrobial agents against Enterococcus faecalis and Candida albicans
Anil Chandra1, Rakesh K Yadav1, Vijay K Shakya1, Suaib Luqman2, Simith Yadav1
1 Department of Conservative Dentistry Endodontics, King George’s Medical University, Lucknow, Uttar Pradesh, India
2 Department of Molecular Bioprospection, Council of Scientific Industrial Research, Central Institute of Medicinal Aromatic Plants (CSIR-CIMAP), Lucknow, Uttar Pradesh, India
Correspondence Address:
Simith Yadav
Department of Conservative Dentistry and Endodontics, King George’s Medical University, Lucknow, Uttar Pradesh
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/denthyp.denthyp_17_17
|
|
|
Introduction: The aim of this ex vivo study was to check the antimicrobial efficacy of silver nanoparticles with and without different antimicrobials against Enterococcus faecalis and Candida albicans. Materials and Methods: Two-hundred and fifty two freshly extracted single-rooted human teeth were contaminated with E. faecalis and C. albicans. The teeth were randomly divided into five experimental (n = 21) and one control group (n = 21). Each subgroup was then exposed to different antimicrobials, namely calcium hydroxide (Ca(OH)2) (Group 1), 2% chlorhexidine (CHX) (Group 2), silver nanoparticles (SNP) (Group 3), SNP with Ca(OH)2 (Group 4), SNP with 2% CHX (Group 5), and saline as control group (Group 6). Cultures were made from each group after 24 h, 7 days, and 14 days, and colony forming units were counted. The Kruskal–Wallis test was used to compare the study parameters among the groups at 24 h, 7 days, and 14 days. Results: Significant difference was found in the antimicrobial efficacy of different intracanal medicaments against E. faecalis and C. albicans after 24 h, 7 days, and 14 days. 2% CHX was found to be the most effective medicament at 24 h, 7 days, and 14 days against E. faecalis and C. albicans. Combination of SNP with 2% CHX and Ca(OH)2 and SNP alone ranked second in their antimicrobial efficacy against E. faecalis and C. albicans at 24 h, 7 days, and 14 days, respectively. Conclusion: 2% CHX was more effective as intracanal medicament against E. faecalis and C. albicans biofilm in both short as well as long-term duration, i.e., at 24 h, 7 days, and 14 days. |
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
